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Welcome to the CyVerse Learning Center (beta)

The CyVerse Learning center is a beta release of our learning materials in the popular “Read the Docs” formatting. We are transitioning our leaning materials into this format to make them easier to search, use, and update. We will be making regular contributions to these materials, and you can suggest new materials or create and share your own. If you have ideas or suggestions please email You can also view, edit, and submit contributions on GitHub. —–

Getting Started

If you are new to CyVerse you may want to join one of our Getting Started webinars, which happen every month or two. You can watch recent webinars on the CyVerse YouTube Channel.

After you Create a CyVerse account, you may want to check out the Platform Guides or the CyVerse FAQ page.

For advise on how to carry out specific tasks or analyses, check out the Quick Starts and Tutorials.

Advanced users can read how to Integrate your own tools and apps or review materials from CyVerse Workshops to reuse in your own teaching.

Platform Guides

CyVerse offers an interconnected series of platforms, tools and services. These guides will help you navigate the top-level user platforms.

Platform/Service Notes Guide Full documentation
Discovery Environment Use hundreds of bioinformatics apps and manage data in the CyVerse Data Store from a simple web interface Discovery Environment Guide DE Manual
Atmosphere Cloud computing platform for CyVerse Atmosphere Guide Atmosphere Manual
Data Store A unified system for managing and sharing your data across CyVerse’s tools and services Data Store Guide Data Store Manual
DNA Subway Educator-focused access to data and informatics tools for modern biology DNA Subway Guide See Guide
BisQue Bio-Image Semantic Query User Environment for the exchange and exploration of image data Coming Soon BisQue Manual
SciApps A web-based platform for reproducible bioinformatics workflows SciApps Guide See Guide
Science APIs CyVerse provides programmatic access to its services through multiple APIs (application programming interfaces), access points with various levels of complexity Agave Live Docs See Live Docs
VICE Visual Interactive Computing Environment VICE introduces graphic user interfaces (GUIs) and common Integrated Development Environments (IDEs) such as Project Jupyter Notebooks & Lab, RStudio, Shiny Apps and Linux Desktop Vice Documentation See Vice Documentation

Quick Starts

These include short guides through common tasks.

Quickstart Platform Notes
Create a CyVerse account User Portal Start here to create your own account
Import data from NCBI SRA using the Discovery Environment Discovery Environment The NCBI Sequence Read Archive (SRA) is a repository for high-throughput sequencing reads. These are valuable data for novel analysis and reuse. You can directly import data from SRA into your Data Store using a Discovery Environment app.
Evaluate High-throughput Sequencing Reads with FastQC Discovery Environment FastQC is a popular tool for evaluating the quality of high-throughput sequencing reads such as from Illumina and PacBio.
Filter, Trim, and Process High-throughput Sequencing Reads with Trimmomatic Discovery Environment Trimmomatic is a popular application for filtering and trimming high- throughput sequencing reads. Several functions can remove populations of low quality reads, remove sequencing adaptors, and trim low-quality regions of individual reads.
EZ installation of popular data science tools Atmosphere and Jetstream Install anaconda (Python 2 or 3, R, Jupyter notebooks), Rstudio, Singularity, or Docker easily on any Atmosphere or Jetstream cloud computer (instance).
Submit High-throughput Sequencing Reads to NCBI Sequence Read Archive (SRA) Discovery Environment The SRA is a canonical repository for sequencing data generated by high-throughput instruments. The CyVerse submission pipeline allows you to directly submit your data into an SRA-linked BioProject.
Request a DOI Data Store, Discovery Environment Organize your dataset and request a DOI (Digital Object Identifier).
Create a Group Project Data Store, Discovery Environment Learn the basic steps for setting up a collaborative project using CyVerse.


These are involved tutorials that cover popular science workflows.

Tutorial Platform(s) Notes
RNA-Seq with Kallisto and Sleuth Discovery Environment, Atmosphere Kallisto is a quick, highly-efficient software for quantifying transcript abundances in an RNA-Seq experiment. Sleuth is designed to analyze and visualize the Kallisto results in R.
Genome Annotation with MAKER SciApps, Discovery Environment This tutorial is a step-by-step guide for using SciApps to perform MAKER based annotation.
Association analysis with mixed models SciApps A genome-wide association study (or GWAS) workflow using TASSEL, EMMAX, and MLMM for mixed model analysis.


These are workshop formatted tutorials that can be used and/or remixed in running your own CyVerse workshop.

Workshop Platform(s) Notes
CyVerse Tools and Services Workshop Discovery Environment, Atmosphere, Data Store This is a generic agenda and slides for a one-day CyVerse Workshop overviewing the major components of the science infrastrutcure.
2018 NEON Data Institute Discovery Environment, Atmosphere Provision Atmosphere as a Data Science Workbench running Docker, Singularity, Project Jupyter, and RStudio-Server. The NEON Data Institute 2018 focus is on remote sensing and reproducible workflows in Python and R.
CyVerse Container Camp 2019 Discovery Environment, Atmosphere, VICE, Data Store Topics on container technology for reproducible science.
Condensed R: 240-minute tutorial Discovery Environment, VICE, Data Store A short introduction to using R and RStudio
CyVerse Foundational Open Science Skills 2019 Workshop to train new PIs on advanced cyberinfrastructure Discovery Environment, Atmosphere, VICE, DataStore

Tool and App Integration

You can contribute to CyVerse - Here are documentation pieces of interest in developing new applications.

Documentation Platform(s) Notes
Vice Documentation VICE Quick guide to developing for VICE.
Creating and Running Docker Containers Discovery Environment, VICE, Atmosphere A short guide to Docker and creating your own containerized applications.

Contributing to the Learning Center

You can contribute to the Learning Center - everything from fixing a typo to adding new documentation pieces.

Tutorial Platform(s) Notes
Documentation Quickstart Learning Center Quick guide to simple contributions and creating new documentation pieces.

CyVerse FAQ

For any and all other questions, check out our CyVerse FAQ.

Get Powered by CyVerse

Third-party projects can leverage our cyberinfrastructure to provide services to their users, including the CyVerse Authentication system, Data Store, and High-performance framework. Projects requesting support by Powered by CyVerse must have the programming resources to integrate with CyVerse APIs and CI, as well as the resources to maintain their sites.

External Collaborative Partnerships

The External Collaborative Partnership program pairs members of our user community with expert staff to address the computational needs of a specific scientific project. To participate, please review the required criteria and then complete the ECP Request web form. CyVerse does not provide funding support for external projects.

About CyVerse

CyVerse Vision: Transforming science through data-driven discovery.

CyVerse Mission: Design, deploy, and expand a national cyberinfrastructure for life sciences research and train scientists in its use. CyVerse provides life scientists with powerful computational infrastructure to handle huge datasets and complex analyses, thus enabling data-driven discovery. Our powerful extensible platforms provide data storage, bioinformatics tools, image analyses, cloud services, APIs, and more.

Originally created under the name iPlant Collaborative as a service to the U.S. plant science communities, CyVerse cyberinfrastructure is relevant to all life sciences disciplines and works equally well on data from plants, animals, or microbes. By democratizing access to supercomputing capabilities, we provide a crucial resource to enable scientists to find solutions for the future. CyVerse is of, by, and for the community, and community-driven needs shape our mission. We rely on your feedback to provide the infrastructure you need most to advance your science, development, and educational agenda.

CyVerse Homepage:

Funding and Citations

CyVerse is funded entirely by the National Science Foundation under Award Numbers DBI-0735191, DBI-1265383, and DBI-1743442.

Please cite CyVerse appropriately when you make use of our resources, CyVerse citation policy.